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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH
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ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15

Issue: June 2024

Volume 9 | Issue 6

Impact factor: 8.15

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Paper Title: A study on detection and characterization of corynebacterium diphtheriae isolated from throat swabs by using manual methods and molecular techniques at a tertiary care hospital
Authors Name: Dr. Gouthami. E , Dr. Shailaja Rani.M , Dr. Shashikala Reddy.P , Dr. Sudha Rani
Unique Id: IJSDR2405013
Published In: Volume 9 Issue 5, May-2024
Abstract: Corynebacterium diphtheriae is an exotoxin producing Gram positive bacillus which causes an upper respiratory tract infection known as Diphtheria. It is a vaccine preventable disease. Even with the successful implementation of universal immunization, the pathogen is reemerging in India due to waning of immunity. The diagnosis of Diphtheria is mainly based on clinical presentation, but the microbiological identification of the pathogen and detection of toxin production is as much as important to prevent the complications of Diphtheria. The current study is conducted at Sir Ronald Ross Institute of Tropical and Communicable diseases, Hyderabad for a period of 2 years from January 2022 to December 2023. Throat swabs were collected from the Patients presenting with clinical features of Diphtheria and processed by microscopy, culture. Biochemical reactions were performed for further identification of the pathogen. Antibiotic sensitivity testing was performed for all the isolates. All the Corynebacterium species were subjected to PCR for identification of toxin production and confirmation of toxin production was done by Modified Eleks test. Total of 1894 throat swab samples were collected during the study period out of which 50 were positive for Streptococcus pyogenes, 37 were positive for Staphylococcus aureus, 50 were positive for Corynebacterium diphtheriae [CD] and 30 were positive for Corynebacterium species other than diphtheriae [COD]. 100% Antibiotic susceptibility was noted for all the isolates. Recent studies have shown that the non-toxigenic toxin bearing strains of Corynebacterium species have been evolved and they can cause milder disease which has shown the importance of booster doses in the adolescents and adults.
Keywords: Corynebacterium diphtheriae, Pharyngitis, Diphtheria, Eleks test, Toxigenic and Nontoxigenic strains
Cite Article: "A study on detection and characterization of corynebacterium diphtheriae isolated from throat swabs by using manual methods and molecular techniques at a tertiary care hospital", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.9, Issue 5, page no.98 - 103, May-2024, Available :http://www.ijsdr.org/papers/IJSDR2405013.pdf
Downloads: 000342048
Publication Details: Published Paper ID: IJSDR2405013
Registration ID:211082
Published In: Volume 9 Issue 5, May-2024
DOI (Digital Object Identifier): https://doi.org/10.5281/zenodo.11256354
Page No: 98 - 103
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631

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